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Direct Research Journals
Direct Research Journal of Biology and Biotechnology:Vol. 3 (5), pp. 74-80, December 2017
ISSN: 4372-2608

Antibacterial activity of the aqueous leaf extract of cassia occidentalis (sanga-sanga) on salmonella typhimurium
Article Number: DRJA10147593
DOI: https://doi.org/10.26765/DRJBB.2017.7593

Original Research Article


1Usman, A. A., *2Bello, A., 4Salihu, A. A., 3Ezeamagu, C. E., 5Odeh, A. P. and 6Zainab, A. B.


1Department of Microbiology, Usmanu Danfodiyo University, Sokoto State, Nigeria.

2Department of Veterinary Anatomy, Usmanu Danfodiyo University, Sokoto State, Nigeria.

3Department of Chemistry, Federal College of Education Technical, Gusau, Zamfara State, Nigeria.

4Department of Agricultural technology, Umaru Ali Shinkafi Polytechnic, Sokoto State, Nigeria.

5Department of Microbiology, Bayero University, Kano, Kano State, Nigeria.

6Microbiology Department, Specialist Hospital, Sokoto, Nigeria.

*Corresponding author E-mail: abccrcfge28@gmail.com


date Received: November 20, 2017     date Accepted: December 8, 2017     date Published: December 12, 2017

Abstract

The Cassia occidentalis leaves was obtained from Usmanu Danfodiyo University Sokoto, Fish farm and it was authenticated at Usmanu Danfodiyo University herbarium after it was taken to Microbiology Laboratory Usmanu Danfodiyo University, Sokoto for aqueous extraction Fifty grams of grounded leaves was dissolved in 500 ml of distilled water and the extract was evaporated in hot air oven at 40°C for 9 days. The concentrations were tested against S. typhi that was obtained from specialist Hospital Sokoto (SHS) after the test isolate was tested for viability and confirmed at the Microbiology Laboratory Usmanu Danfodiyo University, Sokoto. The leaf extract was tested at different concentrations of 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml and 100 mg/ml. But there was no activity recorded at 20 mg/ml. And the minimum inhibitory concentration (MIC) was recorded at 30 mg/ml and minimum bactericidal concentration (MBC) was recorded at 60 mg/ml. The phytochemical screening reveals the presence of Flavonoid (++), Tannin (+), Saponin (+++), Glycoside (+), Cardiac glycoside (+), Steroid (+), Saponin glycoside (+), Anthraquinones (+), and Volatile oil (+). The presence of these photochemical may be attributed to the antibacterial activity of the extracts.


Key words: Antibacterial activity, bacteria, photochemical, salmonella typhimurium, cassia occidentalis