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Direct Research Journals
Direct Research Journal of Agriculture and Food Science:Vol.6 (5), pp. 107-113, May 2018
ISSN 2354-4147

Chrysophyllum albidum seed (African star apple) as an additive in agricultural feed and a potent antimicrobial
Article Number: DRJA35078369
DOI: https://doi.org/10.26765/DRJAFS.2018.8369

Original Research Article


1Akin-Osanaiye B. C., *2Gabriel A. F., 1Salau T. O. and 2Murana O. O.


1Department of Microbiology, Faculty of Science, University of Abuja, Abuja, Nigeria.

2Department of Chemistry, Faculty of Science, University of Abuja, Abuja, Nigeria.

Corresponding author E-mail: yemigabriel@yahoo.com


date Received: April 3, 2018     date Accepted: May 1, 2018     date Published: May 9, 2018

Abstract

This study was aimed at determining the usefulness of Chrysophyllum albidum seed cotyledon as an animal feed additive and the antimicrobial potencies of the seed extracts. Wister Rats, were fed with different percentages of the C. albidum seed mixed with commercial animal feed showed no significant change in their hematological parameters and the histology of the Wister rats’ vital organs. The hematological parameters studied include Part Cell Volume, White Blood Cell, Red Blood Cell, Mean Corpuscular Hemoglobin, Mean Corpuscular Hemoglobin Concentration and differential white blood cell concentration. The antibacterial effect was determined using agar well diffusion assay. Phytochemical analysis of the ethanol extract of C. albidum indicated the presence of flavonoids, tannins, phenols, steroids and saponins. The seed had no adverse effect on the hematological indices of the rat. The maximum activity of ethanol extract was observed on Staphylococcus aureus, which showed clear zones of diameter of 13.0 mm at a Minimum inhibitory Concentration value of 62.5 mg/ml while it had low activity on Pseudomonas aeruginosa, with clear zone of inhibition of 9.0 mm at the same MIC value. The extract, at all concentrations (500 mg/ml, 250 mg/ml, 125 mg/ml and 62.5 mg/ml), showed potent antibacterial activity. The ethanolic extract showed better inhibition against Staphylococcus aureus (ranged from 500 mg/ml to 31.3 mg/ml), P. aeruginosa and E. coli (ranged from 500 mg/ml to 62.5 mg/ml). The Minimum bactericidal concentration of the extract against E. coli was at 500 mg/ml and 250 mg/ml, S. aureus (ranged from 500 mg/ml to 125 mg/ml, and 500 mg/ml) against P. aeruginosa. The potent antibacterial effect of the extract could be attributed to the wide range of active metabolites. Therefore, the seed of C. albidum can be a good source of remedy in phytomedicine and agricultural use.


Keywords: C. albidum, antimicrobial, animal feed, histology, hematology