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Direct Research Journals
Direct Research Journal of Agriculture and Food Science: Vol.5 (6), pp. 257-262, June 2017
ISSN 2354-4147: International Standard Journal Number (ISJN) e-ISJN: A4372-2604

Haematological indices and some biochemical enzymes of Clarias gariepinus (Burchell 1822) fed diets containing Ageratum conyzoides leaf meal
Article Number: DRJA021863974

Original Research Article

Ebochuo V.C.1* and Oparaejiaku, J.2

1Department of Fisheries and Marine Technology, Imo State Polytechnic, Umuagwo, Ohaji, Nigeria.

2Department of Agriculture Management and Economics Technology, Imo State Polytechnic Umuagwo,Ohaji, Nigeria.

*Corresponding author E-mail:

date Received: May 7, 2017     date Accepted: June 16, 2017     date Published: June 23, 2017


This study was conducted to determine the effect of Ageratum conyzoides based diets on the haematology and some biochemical enzymes of Clarias gariepinus juveniles. Ninety fish were assigned to the three experimental diets in which A. conyzoides leaf meal replaced wheat bran at 0%, 50%, and 100% respectively at 30 fish per treatment which was further sub-divided into 3 replicates of ten fish each in a completely randomized design. Range of values for PCV are 20.07%-20.67%, and were not significantly different (P>0.05). A significant WBC value (4.70 ±0.20×103mm3) was produced by 100% A. conyzoides leaf meal diet. RBC was significant (P> 0.05) at 50% and 100% A.conyzoides leaf meal diet over control. HB was similar in all treatments (Range 6.77 ±0.50 – 6.90± 0.30). Lymph level in A. conyzoides based diets were significantly (P<0.05) different from the control fed fish. MCHC, MCH and MCV values were lower than the normal healthy Clarias gariepinus juveniles. ALT, AST values were quite low and do not suggest cellular damage. ALP is however higher in both control and the diets. Results indicate that with proper treatment of A. conyzoides leaf meal, it could be incorporated in fish feed at even 100% with good results.

Key words: Clarias gariepinus, Ageratum conyzoides leaf meal, response, haematology and biochemical enzymes.