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Direct Research Journals
Direct Research Journal of Agriculture and Food Science Vol.1 (4), pp. 52-58, October 2013
ISSN: 2354-4147

Toxicological effects of diazinon as an organophosphate pesticide on fermentation activity of microorganisms and evaluation of sodium bentonite as a toxin binder by using the in vitro batch culture
Article Number: DRJA17089260

Original Research Article

  1. Kazemi1*, A. M. Tahmasbi1, R. Valizadeh1, A. A. Naserian1 and A. Sonei2

1Department of Animal Science, Excellence Center for Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, P. O. Box 91775-1163, Mashhad, Islamic Republic of Iran.

2Plant protection of Pars Taravat, Iran.

date Received: July 16, 2013     date Accepted: August 20, 2013     date Published: October 30, 2013


Rumen microflora may play an important role in biological degradation of organophosphates and other substances with anti-cholinesterase effect. In this experiment the effects of low-term exposure to the diazinon pesticide (0, 100, 250, 500, 750 and 1000 ppm) with sodium bentonite (0, 1 and 2 % of DM) on the ruminal fermentability of microorganisms was investigated using in vitro batch culture. The correlation coefficient between gas production parameters and other fermentation parameters was also investigated. Results indicated that by increasing diazinon to the culture, there were some toxicological effects in the microorganisms activities which lead to the decrease of potential digestibility of DM and other fermentation  parameters such as 96 h cumulative gas production and NH3-N (p<0.05), but the rumen fluid pH was not affected by adding of diazinon. Although sodium bentonite numerically increase the dry matter disappearance rather than control group, however generally it seems rumen fluid couldn’t conceivably be cleaned up the deleterious effects of diazinon at in vitro conditions by sodium bentonite as toxin binder.

Key words: Pesticide, Diazinon, In vitro, Fermentation parameters, Sodium bentonite.