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Direct Research Journals
Direct Research Journal of Agriculture and Food Science:Vol.5 (12), pp. 401-408, December 2017
ISSN 2354-4147

Vegetative Growth Analysis of Hibiscus sabdariffa L. (Roselle) Treated with some Plant Growth Substances
Article Number: DRJA24208379

Original Research Article

*Keshinro, M. O., Kadiri, M., Ojewumi, A. W. and Augustine, T. E.

Department of Pure and Applied Botany, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria.

*Corresponding Author E-mail:

date Received: October 26, 2017     date Accepted: November 20, 2017     date Published: November 27, 2017


Roselle (Hibiscus sabdariffa L.) is a multifunctional plant whose calyx is commonly used for production of indigenous drink called “zobo” in Nigeria. This study was conducted to assess effect of single treatment of 50, 100, 200 mg/l of Gibberellic Acid (GA3 ), Indole Acetic Acid (IAA), 6-Benzylaminopurine (BAP), Naphthalene Acetic Acid (NAA) and 10, 15 and 20 % coconut water on vegetative growth of the plants. Also, effect of combination of 50 mg/L GA3 +10 % coconut water, 100 mg/L GA3 + 15 % coconut water, 200 mg/L GA3 + 20 % coconut water, 50 mg/L GA3 + 50 mg/L IAA, 100 mg/L GA3 + 100 mg/L IAA, 200 mg/L GA3 + 200 mg/L IAA, 50 mg/L GA3 + 50 mg/L NAA, 100 mg/L GA3 + 100 mg/L NAA, 200 mg/L GA3 + 200 mg/L NAA were used. Caffeic acids of 50, 100 and 200 mg/L were evaluated in a completely randomized design. Water served as control. Relative water content (RWC), Relative growth rate (RGR), Net assimilation rate (NAR) and Leaf area ratio (LAR) of the plant investigated were also determined. Data were analyzed using one-way ANOVA and means were separated using Duncan’s Multiple Range Test at p < 0.05. Results showed that highest number of leaves (39.6), leaf area (83.2 cm2) and stem diameter (0.37 mm) were recorded in H. sabdariffa sprayed with 20% coconut water. Also, highest RGR (0.67 gg -1 week -1) was recorded in H. sabdariffa spared with 200 mg/L GA3 and LAR (7541.2 cm2 g -1) in the plant treated with 200 mg/L Caffeic acid while highest NAR (0.3 gg -1 week-1) was recorded in the plants sprayed with combine hormone treatment (200 mg/LGA3+200 mg/LNAA). In conclusion, single hormonal treatments were the best in improving vegetative parameters of H. sabdariffa .

Key words: Hibiscus sabdariffa, vegetative growth, growth substance, relative water content, net assimilation rate, leaf area ratio.