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Direct Research Journals
Direct Research Journal of Health and Pharmacology (DRJHP):Vol. 5 (3), pp. 14-23, December 2017
ISSN 2449-0814

Proteomic identification of larval excretory/secretory diagnostic biomarkers for early hyperinfection in strongyloidiasis
Article Number: DRJA26047842
DOI: https://doi.org/10.26765/DRJHP.2017.7842

Original Research Article


Aliyu Mahmuda1*, Al-Zihiry Khalid Jameel Kadhim2, Zasmy Unyah3,  Roslaini Abd Majid3, Rukman Awang Hamat3, Yusuf Yakubu4, Abdurrahman Bello5, Abdullahi Abdullahi Raji6, Umar Salisu Ahmad7 and  Yahaya Abubakar Yabo8


1Department of Parasitology and Entomology, Faculty of Veterinary Medicine, Usmanu, Danfodiyo University,

Sokoto, Nigeria.

2Department of Microbiology, College of Medicine, University of Thi-Qar, Iraq.

3Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia.

4Department of Public Health and Preventive Medicine, Faculty of Veterinary Medicine, Usmanu, Danfodiyo University, Sokoto, Nigeria.

5Department of Anatomy, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.

6Department of Pathology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.

7Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.

8Department of Physiology and Biochemistry, Faculty of Veterinary Medicine, Usmanu Danfodiyo University,

Sokoto, Nigeria.

*Corresponding author E-mail: alibadra1213@gmail.com


date Received: November 20, 2017     date Accepted: December 9, 2017     date Published: December 11, 2017

Abstract

The aim of the current study was to identify early specific diagnostic proteins from the components of Strongyloides ratti larval Excretory/Secretory (E/S) products. Products from the treated (Prednisolone) and non-treated (control) groups were analysed using two dimensional (2D) gel electrophoresis, LC-MS/MS and MALDI-TOF/TOF Mass Spectrophotometry. A total of 10 different protein biomarkers were detected as overexpressed from the ES treated products with molecular weights ranging from 30 – 90 kDa and isoelectric points (IP) range from 4 – 9. Eight (8) overexpressed protein biomarkers were also identified from the infected and prednisolone-treated rat sera. Relative quantitative real-time PCR (qPCR) was performed to compare the expression level of the biomarker genes between the treated and non-treated groups in order to validate the related expressed biomarkers. Nine (9) related genes were observed to show significant high expression levels of the identified biomarkers, whereas one (1) gene (Arg) recorded down regulation in its expression. The identified proteins in this study might be of specific importance as diagnostic biomarkers for early hyperinfection syndrome in strongyloidiasis.


Key words: Proteomics, diagnostic biomarkers, hyperinfection, strongyloidiasis