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Direct Research Journals
Direct Research Journal of Agriculture and Food Science (DRJAFS) Vol.3 (8), pp. 157-161, August 2015
ISSN: 2354-4147

Isolation, characterization and identification of effective microorganisms from Malaysia biofertilizer products
Article Number: DRJA17085319

Original Research Article

*G.H. Tan and S. A. Nazaruddin

Department of Agriculture Technology, Faculty of Agriculture, University of Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

date Received: July 23, 2015     date Accepted: August 2, 2015     date Published: August 11, 2015


Nowadays, the use of biofertilizer in agriculture is an alternative approach in sustainable farming in order to sustain the balance of natural ecosystem. There are various biofertilizer products in Malaysia market that claimed to have effective and beneficial microorganisms possibly to enhance the plant growth. Therefore, the purpose of this study was to isolate, characterize and identify the effective microorganisms from biofertilizer and also to find out its functionality and present. Seven different microbes were successfully isolated from four types of biofertilizer products: sample SR1 Bunga, sample SG-1, sample SKT-1 and sample SKT-2. Results from gram staining showed that all isolates were gram positive bacteria. Three types of tests were conducted to identify their functionality. Nitrogen fixation ability test was carried out using nitrogen-free media by observing the colour changes of growth media around the bacteria colony from green to blue. Phosphate solubilization test was conducted using Pikovskaya’s agar media by observing the formation of clear halo zone, while the Indole acetic acid (IAA) production was carried out by adding the tryptophan and checked the optical density of the colour changes in the broth media. These isolates were identified by 16S rDNA determination. Bacillus and Staphylococcus groups are the most dominant strain can be found in these products, These bacteria have potential to fix atmospheric nitrogen, able to produce IAA with the range of 15.13± 0.2 to 33.1± 0.2 µg/ml when supplemented with 100 µg/ml of tryptophan, and showed some P-solubilizing activity which have been claimed by manufacturers.

Key words: Biofertilizer, isolation, indole acetic acid, (IAA), nitrogen fixation, phosphate solubilization