Original Research Article
Antimicrobial Effects of Lauric Arginate and Ethylenediaminetetraacetic Acid against Salmonella Typhimurium and Salmonella Enteritidis Inoculated on Skinless Ground Chicken Breast Stored at 3±1⁰C
Clarissa M. Harris |
Sally K. Williams* |
Article Number: DRJAFS10600889
DOI: https://doi.org/10.26765/DRJAFS10600889
ISSN: 2354-4147
Vol.8 (6), pp. 191 -198, June 2020
Copyright © 2020
Author(s) retain the copyright of this article
Abstract
The objectives of this research were to determine antimicrobial effects of Lauric arginate (LAE) alone and in combination with Ethylenediaminetetraacetic acid (EDTA) against Salmonella Typhimurium (ST), Salmonella Enteritidis (SE), and total psychrotrophs in ground chicken meat, and the effects of LAE and EDTA on pH of the ground meat. Salmonella Typhimurium (107 cfu/g) and Salmonella Enteritidis were inoculated separately into ground chicken breast meat and treated with LAE alone (0.1, 0.5, 1.0, and 2.0%) and in combination with 0.25% EDTA and stored for 7 days at 3.0 ± 1°C. This study was repeated two times. LAE at 0.1% alone and in combination with EDTA reduced (< 0.05) ST on days 0, 1 (0.1% LAE plus EDTA only on day 1) and 7, and reduced psychrotrohic counts on days 5 and 7 when compared to all other treatments. 1% (except for day 3) and 2% LAE alone, and 0.5, 1, and 2% LAE in combination with EDTA had lower (< 0.05) ST on all days when compared to positive control; and psychrotrophs were reduced when treated with LAE and EDTA combinations, and 1 and 2% LAE alone on day 7. LAE and EDTA had minimal effects on pH for SE, and decreased for ST when combinations of LAE and EDTA were employed. In this study, EDTA and LAE combinations created synergistic effects in reducing bacteria to lower levels than when either EDTA or LAE was used alone. Unlike S. Typhimurium, S. Enteritidis was not as sensitive to LAE and EDTA.
Keywords: Salmonella Typhimurium, psychrotrophic bacteria, lauric arginate, microbiology, shelf lifeReceived: May 10, 2020 Accepted: June 16, 2020 Published: June 30, 2020