Article Number: DRJAFS22091758

DOI: https://doi.org/10.26765/DRJAFS22091758

ISSN: 2354-4147

Vol. 9, Pp. 77-86, 2021

Copyright © 2021

Author(s) retain the copyright of this article


Original Research Article

Assessing the genetic variability of cowpea (Vigna unguiculataL.) genotypes using phenotypic traits and SDS markers

Magda N. Rajab, Mervat,*

R. I., Sayed

M. A. Abd El- Monem


Abstract

Cowpea (Vigna unguiculata L.) is one of the most important pulse crops which serve multiple purposes for human food and animal feed. Information on genetic diversity among the existing cowpea genotypes will increase the efficiency of the cowpea improvement. The present investigation was carried out at the experimental farm of Sids Agriculture Research Station, ARC, during the 2017 and 2018 summer seasons. A total of seventeen cowpea genotypes were separately sown in a randomized complete block design (RCBD) with three replications to detect the magnitude of variability and the degree of association between the different traits based on the performance of yield and its yield components and seed storage protein. The combined analysis over the two years revealed that significant differences were found among the cowpea genotypes for all studied traits. Genotype 4 ranked first followed by genotype 6 for the highest values of yield and its components over the two seasons and combined, indicating that these genotypes are promising and could be recommended to use among breeding programs. The great variation was recorded with the obtained values of genetic and phenotypic coefficient of variation, heritability, and genetic advance for all phenotypic traits. Highly significant positive correlation values were found between dry forage yield and each plant height, stem diameter, and fresh yield, while a significant negative correlation with leaf/ stem ratio at both the genotypic and phenotypic levels. These are useful for simultaneous selection for yield improvement in cowpea. Twenty-four bands were detected with the molecular masses ranging from 17 up to 245 kDa in seventeen cowpea genotypes by SDS- PAGE electrophoresis patterns for water-soluble proteins. Based on seed protein combined analysis, the overall similarity matrix revealed that the highest similarity between genotypes 9 and 10 was (93.7%) while the lowest similarity was (8.1%) between genotypes 1 and 16. The dendrogram resulting from the combination of the two systems separated the seventeen cowpea genotypes into two main clusters and two sub clusters. This study provided baseline information and identified promising cowpea genetic resources for effective breeding and systematic conservation.


Keywords: Cowpea, heritability, genetic advance, SDS- PAGE electrophoresis


 Received: February 17, 2021  Accepted: March 27, 2021  Published: April 6, 2021

Magda Et Al


Copyright © 2021 Direct Research Journal of Agriculture and Food Science