Article Number: DRJA36074545


ISSN: 2354-4147

Vol.4 (7), pp. 176-181, July 2016

Copyright © 2016
Author(s) retain the copyright of this article

Original Research Article

Red (Brassica oleracea var. capitata) and White (Brassica oleracea var. botrytis) Cabbage Leaves Nutritional Value as Forage Feed: Comparison Study of In Vitro Gas Production and Determination of Chemical Composition

Mohsen Kazemi1*

Ameneh Eskandary Torbaghan2

Elias Ibrahimi Khoram Abadi1,

Saeid Abdi Tazik1

Reza Tohidi1


An in vitro rumen gas production technique was used to compare chemical composition and nutritive value of red (Brassica oleracea var. capitata) and white (Brassica oleracea var. botrytis) cabbage leaves (Brassicaceae). There were no significant differences in either extract concentration, but other chemical composition (such as Neutral detergent fiber: NDF; Acid detergent fiber: ADF; Ash, and Dry matter concentrations) was significantly higher for red cabbage leaves (RCL) and the crude protein (CP) concentration was higher (p<0.05) for white cabbage leaves (WCL). Total gas produced at 24, 48 h and Bgas (gas production from the insoluble but fermentable fraction; ml)  was significantly highest for WCL than to RCL (p<0.05). The highest (P<0.05) estimated parameters from in vitro gas production values (Organic matter digestibility: OMD; Metabolisable energy: ME; Short chain fatty acid: SCFA, and microbial protein yield) were observed for WCL. In vitro rumen gas production can be used as a method to distinguish differences in nutritive value of forages like this study. A difference between two forage species in chemical composition is responsible for the fermentation pattern. Fresh agricultural waste, particularly leaves from RCL and WCL, should be a suitable feed for ruminants, although it seems that the nutritional value of WCL is better than RCL.

Key words: Brassicaceae, in vitro, chemical composition, gas production.



Akinfemi A (2012). Evaluation of nutritive value of vegetable wastes grown in north central nigeria using in vitro gas production technique in ruminant animals. J. anim. Sci. adv. 2(12): 934-940.
AOAC (1990). Official Methods of Analysis, 15th ed., Association of Official Analytical Chemists, Arlington, VA, USA.
Babayemi OJ, Demeyer O, Fievz V (2004). In vitro fermentation of tropical browse seeds in relation to their content of secondary metablites. J. Anim. Feed Sci. 13: 31-34.
Cartea ME, Velasco P (2008). Glucosinolates in Brassica foods: bioavailability in food and significance for human health. Phytochem. Rev. 7(2): 213-229.
Cassida KA, Barton BA, Hough RL, Wiedenhoeft MH, Guillard K (1994). Feed intake and apparent digestibility of hay-supplemented Brassica diets for lambs. J. Anim. Sci. 72:1623-1629.
Chumpawadee S, Chantiratikul A, Chantiratikul P (2007). Chemical compositions and nutritional evaluation of energy feds for ruminants using in vitro gas production technique. Pak. J. Nutr. 6 (6): 607-612.
Czerkawski JW (1986). An introduction to rumen studies. Pergamon Press. Oxford. New York.
De Ruiter JM, Dalley DM, Hughes TP, Fraser TJ, Dewhurst RJ (2007). Types of supplements: Their nutritive value and use. pp. 97-115. In: Pasture and supplements for grazing animals. Eds. Rattray PV, Brookes IM, Nicol AM, New Zealand Society of Animal Production Occasional Publication No. 14. El- Khoderi S, El-Boshy M, Gaafar KH, Elmashad A (2007). Hypocalcaemia in Ossimi sheep associated with feeding on beet tops (Beta vulgaris). Turk. J. Vet. Anim. Sci. 32(3): 199-205.
Fahey JW, Zalcmann AT, Talalay P (2001). The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochem. 56(1): 5-51.
Freyman S, Toivonen PM, Perrin PW, Lin WC, Hall JW (1991). Effect of nitrogen fertilization on yield, storage losses and chemical composition of winter cabbage. Can. J. Plant Sci. 71:943-946.
Garcia MA, Aguilera JF, Molina E (2005). Ruminal degradability and in vitro intestinal digestibility of sunflower meal and in vitro digestibility of olive byproducts, supplemented with urea or sunflower meal. Livest. Prod. Sci. 44: 245 “255.
Getachew G, Blummel M, Makkar HPS, Becker K (1998). In vitro gas measuring techniques for assessment of nutritional quality of feeds: A review. Anim. Feed Sci. Technol., 72: 261-281.
Hartnell GF, Hvelplund T, Weisbjerg MR (2005). Nutrient digestibility in sheep fed diets containing Roundup ready or conventional fodder beet, sugar beet, and beet pulp. J. Anim. Sci. 83: 400-407.
Khan MI, Atreja PP (2005). Capturing energy from agro-industrial residues: turning waste into value added products. Small Rumin. Res. 64: 279-284.
Khattab HM, Kholif AM, El-Alamy HA, Salem FA, El-Shewy AA (1999). Ensiled banana wastes with molasses or whey for lactating buffaloes during early lactation. Asian-Aust. J. Anim. Sci. 13 (5): 619-624.
Makkar HPS (2005). In vitro gas methods for evaluation of feeds containing phytochemicals. Anim. Feed Sci. Technol.123-124: 291-302.
Marimo CT, Hector B, Rodgrigues PHM, BLM, Meyer PM, Alves da Silva EJ, Orskov ER (2010). Characterization of vegetables and fruit potential as ruminant feed by in vitro gas production technique. LRRD. 22: Article #168.
Mauricio RM, Mould FL, Dhanoa MS, Owen E, Channa KS, Theodorou MK (1999). A semi-automated in vitro gas production technique for ruminant feed stuff evaluation. Anim. Feed Sci. Technol. 79: 321“330.
Mebrahtu O, Tenaye SB (1997). Analytical methods for Feeds Animal Excrements and Animal Tissues. International Livestock Research Institute (ILRI) Nutrition Laboratory ILRI-Ethiopia, Addis Ababa, Ethiopia.
Menke KH, Raab L, Salewski A, Steingass H, Fritz D, Schneider W (1979). The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro. J. Agric. Sci. (Cambridge) 92:217-222.
Menke K, Steingass H (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28: 7-55.
Nguyen TN, Ledin I (2005). Effects of feeding wastes from Brassica species on growth of goats and pesticide / insecticide residues in goat meat. Asian Australas. J. Anim. Sci. 18(2): 197-202.
Nkosi BD (2010). Effect of dietary inclusion of discarded beetroot and potato hash silage on growth performance and digestibility in South African dorper lambs. J. Anim. Sci. Vet. Adv. 9:853-856.
Oni AO, Onwuka GFI, Oduguwa OO, Onifade OS, Arigbede OM (2008). Utilization of citrus based diets and Enterolobium cyclolarpunt. (JACQ GRISEB). Foliage by West African dwarf goats. Livest. Sci. 117, 184-191.
Ørskov ER, McDonald I (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J Agric Sci 92: 499-503.
Reid RL, Puoli JR, Jung GA, Cox-Ganser JM, McCoy A (1994). Evaluation of Brassicas in grazing systems for sheep: quality of forage and animal performance. J. Anim. Sci. 72: 1823-1831.
Theodorou MK, Williams BA, Dhanoa MS, Mc Allan AB, France J (1994). A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim. Feed Sci. Techol. 48: 185“197.
Tobias MC, Hector CB, Mazza PH, Ridrigues LM, Oliveria P, Marques M, Alves da Silva EJ, Ørskov ER (2010). Characterization of vegetables and fruits potential as ruminant feed by in vitro gas production technique. LRRD. 22 (9). See in:
Ulyatt MJ, Fennessy PF, Rattray PV, Jagusch KT (1980). The nutritive value of supplements. In: Supplementary feeding. Eds. Drew, K.R., Fennessy, P.F. New Zealand Society of Animal Production Occasional Publication No. 7.
Van Soest PJ, Roberson JB, and Lewis BA (1991). Methods for dietary fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.
Wadhwa M, Kaushal S, Bakshi MPS (2006). Nutritive evaluation of vegetable wastes as complete feed for goat bucks. Small Rumin. Res. 64: 279-284.




Dr. G.A. Pesewu
Department of Medical Laboratory Sciences (MEDLAB), School of Biomedical and Allied Health Sciences, College of Health Sciences, University of Ghana, PO Box KB 143, Korle-Bu, Accra, Ghana.



 Received: April 18, 2016  Accepted: June 14, 2016  Published: June 18, 2016


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