Article Number: DRJA11319565


ISSN: 2354-4147

Vol.4 (7), pp. 137-143, July 2016

Copyright © 2016
Author(s) retain the copyright of this article

Original Research Article

Efficacy of Smart Fertilizer for Combating Bacterial Wilt Disease in Solanum Lycopersicum

M. Z. A. Radhi1,3

M. B. Adam2

H. M. Saud1

M. N. Hamid3

P. S. H. Tony4

G. H. Tan1*


Tomato plants are susceptible to bacterial wilting, which causes production losses varying from 10 to 100%. In Malaysia, there are more than 35 families of plants are affected by this disease, and the major economic host including potato, tomato, eggplant, chili, ginger and groundnut.  Although there was no published data on the exact losses in Malaysia caused by Ralstonia solanacearum, however, this disease caused tomato yield losses up to 70 % of farmers income.  This work had the objective of evaluating œsmart fertilizer for controlling and/or preventing bacterial wilt disease and to study the overall growth of tomato plants in control environment. The plants height was increased from week-4 to week-12 with the average of 104.92 cm in treatment T1 (formulated phage fertilizer); 93 cm in treatment T2 (fertilizer alone) and only 43.9 cm in treatment T3 (common commercial fertilizer). The growth performance of tomato plants was dwarfed in T3. There are similar results in stem observation, fresh weight, dry weight and total fruits produced. The total fruits yield in T3 was lesser than one third in both T1 and T2 due to a very high mortality rate in T3 by wilting especially at the fruiting stage of the plants . Finally, we concluded that the used of smart fertilizer can be used as an alternative for the bacterial wilt control.

Key words: Ralstonia solanacearum, smart fertilizer, bacterial wilt, Solanum lycopersicum, bacteriophage.



Abdullah H (1992). Bacterial wilt in Malaysia: hosts, disease incidence and geographical distribution. Proceedings in the International bacterial wilt symposium, Kaoshiung, Taiwan, P.8.
Akhtar M, Malik A (2000). Roles of organic soil amendments and soil organisms in the biological control of plant-parasitic nematodes: A review. Bioresour Technol. 74:35-47.
Agrios GN (2005). Plant pathology 5th Edition. Academic Press, San Diego, CA. P.952.
Bailey KL, Lazarovits G (2003). Suppressing soil-borne diseases with residue management and organic amendments. Soil Till Res. 72:169“180.
Cook RJ, Baker KF (1983). The nature and properties of plant pathogens. American Phytopathological Paul, MN. P. 539.
Denny TP, Baek SR (1991). Genetic evidence that extracellular polysaccharide is a virulence factor of Pseudomonas solanacearum. Mol. Plant-Microbe Interact. 4:198-206.
DAO, 2000. Department of Agriculture Malaysia. Annual Report. P.120.
Elphinstone JG (2005). The current bacterial wilt situation: a global overview, p. 9“28. In: C. Allen, P. Prior and A.C. Hayward (ed.), Bacterial Wilt Disease and the Ralstonia solanacearum species Complex. American Phytopathological Society Press, St. Paul, MN. P. 9“28.
Genin S, Boucher C (2002). Ralstonia solanacearum: Secrets of a major pathogen unveiled by analysis of its genome. Mol Plant Pathol. 3:111“118.
González ET, Allen C (2003). Characterization of a Ralstonia solanacearum operon required for polygalacturonate degradation and uptake of galacturonic acid. Molecular plant-microbe interactions. 16(6):536-544.
Hamidah S, Lum KY (1992). Bacterial wilt of groundnuts in Malaysia. In: Hartman GL, Hayward AC, ed., Bacterial wilt. ACIAR Proceedings 45: P. 225-227.
Hartman GL, Hong WF, Wang TC (1991). Survey of bacterial wilt on fresh market hybrid tomatoes in Taiwan. Plant Prot. Bull. 33:197-203.
Hayward AC (1991). Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29: 65-87.
Hayward AC (2000). Ralstonia solanacearum. In Joshua Lederberg (Ed.), Encyclopedia of microbiology, San Diego, CA: Academic Press. P32- 42.
Hyakumachi M, Nishimura M, Arakawa T, Asano S, Yoshida S, Tsushima S, Takahashi H (2013). Bacillus thuringiensis suppresses bacterial wilt disease caused by Ralstonia solanacearum with systemic induction of defense-related gene expression in tomato. Microbes Environ. 28:128“134.
Kang Y, Liu H, Genin S, Schell MA, Denny TP (2002). Ralstonia solanacearum requires type 4 pili to adhere to multiple surfaces and for natural transformation and virulence. Molecular Microbiology 46(2): 427-437.
Kurozawa C, Pavan MA (2005). Doenças do tomateiro (Lycopersicon esculentum) In: KIMATI, H. et al. (Ed.). Manual de fitopatologia. São Paulo: Ceres. 2:(67):607-626.
Kurtboke I (2012). Bacteriophages. 1st Edition. In Tech Press, Croatioa. P.268.
Lopes CA and Santos JRM (1994). dos. Doenças do tomateiro. Brasília: Embrapa-SPI/Embrapa-CNPH, P.67.
López MM, Biosca EG (2005). Potato bacterial wilt management: new prospects for an old problem, p. 205“224. In: C. Allen, P. Prior, and A.C. Hayward (ed.), Bacterial Wilt Disease and the Ralstonia solanacearum species Complex. American Phytopathological Society Press, St. Paul, MN. P. 205“224.
Masyitah (2004). Development of disease suppressive compost and potting mix for control of bacterial wilt of tomato. MSc. Thesis, Universiti Putra Malaysia. P. 24.
Mansfied J, Genin S, Magor S (2012). Top 10 plant pathogenic bacteria in molecular plant pathology. Mol. Plant Pathol. 13:614“629.
Peixoto AR (1997). Biological control of bacterial wilt of tomato by fluorescent Pseudomonas spp. Ciência Rural, Santa Maria, 27(1):153-160.
Ray DK, Mueller ND, West PC, Foley JA (2013). Yield trends are insufficient to double global crop production by 2050. PLoS One 8:e66428.
Salanoubat M, Genin S, Artiguenave F, Gouzy J, Mangenot S, Arlat M, Billault A, Brottier P, Camus JC, Cattolico L, Chandler M (2002). Genome sequence of the plant pathogen Ralstonia solanacearum. Nature 415(6871):497-502.
Silveira NSS, Mariano RLR, Michereff SJ (1996). Pseudomonas solanacearum no Brasil. Summa Phytopathologica, Jaboticabal, 22 (2): 97-111.
Tahat MM, Sijam K, Othmann R (2012). The potential of endomycorrhizal fungi in controlling tomato bacterial wilt Ralstonia solanacearum under glasshouse conditions. Afr. J. Biotechnol. 11:13085“13094.
Tan GH, Nordin MS, Napsiah AB, Rosnah H (2009). The lysis activity of bacteriophages isolated from sewage against Ralstonia solanacearum and Erwinia chrysanthemi. J. Trop. Agric. Food. Sc. 37(2):203“209.
Tan GH, Nordin MS, Napsiah AB (2010). The effect of infection on pathogenic activity of Ralstonia solanacearum in tomato. J. Trop. Agric. Food. Sc. 38 (1): 123-130.
Tans-Kersten J, Huang H, Allen C (2001). Ralstonia solanacearum needs motility for invasive virulence on tomato. J. Bacteriol. 183(12): 3597-3605.
Takahashi H, Nakaho K, Ishihara T, Ando S, Wada T, Kanayama Y, Asano S, Yoshida S, Tsushima S, Hyakumachi M (2014). Transcriptional profile of tomato roots exhibiting Bacillus thuringiensis-induced resistance to Ralstonia solanacearum. Plant Cell Reports 33:99“110.
USDA (2003). Biological control of Fusarium wilt and other soil-borne pathogenic fungi.
Wang JF, Lin CH (2005). Integrated management of tomato bacterial wilt. AVRDC-The world vegetable center, Taiwan. P. 615.
Whipps J (2001). Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot. 52:487“511.
Yabuuchi E, Kosako V, Yano I, Hotta H, Nishiuchi Y (1995). Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia picketii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol Immunol. 39:897“904.
Yamada T, Kawasaki T, Nagata S, Fujiwara A, Usami S, Fujie M (2007). New bacteriophages that infect the phytopathogen Ralstonia solanacearum. Microbiology 153: 2630“2639.
Youssef SA, Tartoura KAH. (2013). Compost enhances plant resistance against the bacterial wilt pathogen Ralstonia solanacearum via up-regulation of ascorbate-glutathione redox cycle. Eur. J. Plant Pathol. 137:821“834.
Yuliar YAN, Nion YA, Toyota K (2015). Bacterial wilt disease caused by Ralstonia solanacearum. Microbes Environ. 30 (1): 1-11.


Manuscript reviewed by:


Prof. Adel Shatta
Food Technology Department,Faculty of Agriculture,Suez Canal University.
Dr.Elena Delian
USAMV Bucharest, Romania,Plant Physiology.
Dr. Jolyon Dodgson
Faculty of Science,Mahasarakham University,Kantarawichai District
Maha Sarakham Province,Thailand.
Dr. Ernest Eteng
Department of Soil Science & Meteorology, MOUA, Umudike Abia State, Nigeria.

 Received: May 12, 2016  Accepted: May 26, 2016  Published: June 18, 2016


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