Article Number: DRJAFS41886447
DOI: https://doi.org/10.26765/DRJAFS41886447
ISSN: 2354-4147

Vol. 10(3), Pp. 134-138, May 2022

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Original Research Article

Understanding the Antimicrobial Effects of Lauric Arginate, Ethylenediaminetetraacetic Acid (EDTA), and a Vinegar Based Ingredient against Salmonella Spp. using Transmission Electron Microscopy and Flow Cytometry

Clarissa M. Harris*

Sally K. Williams


Abstract

Transmission electron microscopy inspection of lauric arginate, a vinegar-based ingredient, and lauric arginate in combination with EDTA did not show any disruption of the cytoplasmic membrane. Instead, all of the micrographs showed an intact cell with no leakage compared to the negative control. This study indicated that more work needs to be conducted and an alternative method needs to be evaluated in order to evaluate the cell morphology. When staining cells with PI and SYTO9, the membrane was affected.  Results showed that there was a population of dead cells distinguished from the live cells. Although you can distinguish the populations, the density of the area represents only a certain quadrant of the total volume of the cells.


Keywords: Antimicrobial mechanism, salmonella, cells


 Received: February 13, 2022  Accepted: March 18, 2022  Published: May 21, 2022


Copyright © 2022 Direct Research Journal of Agriculture and Food Science