Article Number: DRJHP22408976325

DOI: https://doi.org/10.26765/DRJHP22408976325

ISSN: 2449-0814

Vol.8 (6), pp. 102-108, October 2020

Copyright © 2020

Author(s) retain the copyright of this article


Original Research Article

Biochemical Assessment of the Effects of Aqueous Stem Bark Extract of Detarium Microcarpum on Wistar Strain Rats

Bello, M. I.*

Shuaibu, H.


Abstract

This study revealed the presence of alkaloids, tannins, saponins, phenols, phlobatannins, and flavonoids when the aqueous stem bark extract of Detarium microcarpum was subjected to qualitative phytochemical screening. Quantitatively, alkaloids (1.32±0.02); tannins (0.42±0.01); saponins (20.45±0.46); phenols (1.86±0.06) and flavonoids (0.39±0.03) were estimated. Antimicrobial susceptibility test of the aqueous stem bark extract using Escherichia coli, Neisseria gonorrhoeae, Staphylococcus aureus, and Klebsiella pneumoniae as test organisms showed varying zones of inhibition with 10mm zone of inhibition of the growth of Staphylococcus aureus exhibited by the extract which corresponded to that exhibited by ofloxacin for the same bacteria. Similarly, the 15mm zone of inhibition of the growth of Neisseria gonorrhoeae exhibited by the extract corresponded with that exhibited by ampicillin for the same organism. Minimum Inhibitory Concentration (MIC) was 16µg/ml for both Staphylococcus aureus and Neisseria gonorrhoeae and 32µg/ml for both Escherichia coli and Klebsiella pneumoniae. Minimum Bactericidal Concentration (MBC) of the extract was 64µg/ml for Staphylococcus aureus and none for the remaining three test organisms.  Treatment of experimental animals with the aqueous stem bark extract for a period of six weeks and subsequent assessment of its effect biochemically showed that aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) levels were significantly higher than normal suggesting certain degree of liver injury. Acid phosphatase and alkaline phosphatase levels were significantly lower (p<0.05) as compared to normal. Glucose, cholesterol, total bilirubin, and direct bilirubin levels were significantly lower than normal while albumin and total protein levels were significantly higher (p<0.05) than normal. Urea and creatinine levels were significantly higher (p<0.05) than normal/control values indicating some degree of kidney injury.


Keywords: Detarium microcarpum, aqueous stem bark extract, zone of inhibition, aspartate and alanine aminotransferases, and test organisms


 Received: September 6, 2020  Accepted: October 13, 2020  Published: October 30, 2020

Bello And Shuaibu


Copyright © 2020 Direct Research Journal of Health and Pharmacology