Article Number: DRJA17085310
Vol.4 (1), pp. 1-7, January 2016
Copyright © 2016
Author(s) retain the copyright of this article
Original Research Article
Effect of methanol leaf extract of anogeissus leiocarpus on gentamicin induced biochemical derangement in rats
In traditional medicine, different parts of Anogeissus leiocarpus are highly valued for the treatment of array of human diseases including cardiotonic, diuretic, hepatoprotective, antimicrobial and anti-inflammatory activity. In the present study, single oral dose (100-400 mg/kg/day for 7 days) of methanol leaf extract of A. leiocarpus leaves were studied for its hepatoprotective and nephroprotective effect on albino rats. In the study of gentamicin induced abnormalities in metabolic biochemical parameters in albino rats, thirty six wistar albino rats were evenly divided into six groups of six animals each. The treated groups received gentamicin and doses of (100, 200 and 400 mg/kg) body weight per day of leaf extract respectively and were compared with the control group. On the 8th day, the blood samples of all the groups were taken for biochemical examination on different parameters that is, serum levels of urea, protein, creatinine, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase, total bilirubin and direct bilirubin, as well as quantitative ionic estimation of some ions like sodium, potassium, chloride and bicarbonate. In the study of gentamicin induced abnormalities in rats treated with extract (100-400 mg/kg) significantly (p<0.05) decreased the levels of aspartate transaminase (AST) from 182.72± 12.43(U/L) to 94.41±3.25 (U/L) , alanine transaminase (ALT) from 68.03±3.51 (U/L) to 35.14±8.88 (U/L), alkaline phosphatase (ALP) from 278.12±4.67 (U/L) to 245.39±12.02 (U/L), serum creatinine from 115.77±2.75(mmol/L) to 62.77±5.23 (mmol/L), acid phosphatase from 336.00±33.27 (U/L) to 240.62±34.28 (U/L) and serum urea from 8.39±0.95 (mmol/L) to 2.94±0.32 (mmol/L) when group 2 is compared with group 6h owever, the plant extract reversed the effect of gentamicin in potassium ion from 12.64±0.68 (mmol/L) to 13.32 ± 0.86(mmol/L), chloride ion from 80.09+1.99(mmol/L) to 87.13±1.95 (mmol/L) and bicharbonate ion from 25.00±0.49 (mmol/L) to 25.21±0.37 (mmol/L) when group 2 was compared with group 6. The results suggest that Anogeissus leiocarpus is a potent hepatoprotective and nephroprotective agent which is capable of normalizing biochemical abnormalities associated with liver and kidney damage.
Key words: Anogeissus leiocarpus, gentamicin, aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase.