Article Number: DRJHP721763058

DOI: https://doi.org/10.26765/DRJHP721763058

ISSN: 2449-0814

Vol.8 (5), pp. 66-79, August 2020

Copyright © 2020

Author(s) retain the copyright of this article


Original Research Article

Effects of Crude and Degummed Seed Oil Extracts of Citrullus lonatus on Carbon Tetrachloride (CCl4) Induced Liver Damage in Wistar Rats

*Bello, M. I.

Tijwun, T. L.


Abstract

Qualitative and quantitative phytochemical screening, antioxidant activity, biochemical and haematological effects of crude and degummed seed oil extracts of Citrullus lonatus on carbon tetrachloride-induced liver damage in rats were determined. Qualitative phytochemical screening showed the presence of saponins, terpenoids, flavonoids, alkaloids and steroids in both crude and degummed seed oil extracts while tannins, glycosides and phenols were absent in both samples. Quantitatively, saponins (4.02 ± 0.01 and 2.60 ± 0.01), terpenoids (1.71 ± 0.06 and 2.17 ± 0.05), flavonoids (2.91 ± 0.01 and 3.49 ± 0.02), alkaloids (1.11 ± 0.01 and 0.74 ± 0.01) and steroids (5.13 ± 0.01 and 4.43 ± 0.01) were determined for the crude and degummed seed oil extracts respectively. Crude and degummed seed oil extracts possessed antioxidant activities compared to L-ascorbic acid. The results were expressed in percentage inhibition (IC50) (mg/ml). Degummed seed oil extract exhibited better antioxidant activity with IC50 38.22 mg/ml, 38.66 mg/ml and 39.32 mg/ml for FRAP, DPPH and TBARS compared to crude seed oil extract 38.38 mg/ml, 38.86 mg/ml, 39.39 mg/ml and L-ascorbic acid 41.25 mg/ml, 48.14 mg/ml and 58.15 mg/ml respectively. Crude and degummed seed oil extracts at 100 to 300 mg/kg body weights both exhibited their ameliorative effects on the deleterious effects of carbon tetrachloride on the experimental animals by significantly (p< 0.05) decreasing serum levels of ALT, AST, ALP TC, TG and LDL and significantly (p<0.05) increasing total protein, albumin, bilirubin and HDL levels. Haematological indices observed showed that the crude and degummed seed oil extracts at 100 to 300 mg/kg body weights significantly (p< 0.05) decrease the levels of WBCs, MCV, MCH and MCHC while RBC, PCV, Hb and PLT levels significantly (p< 0.05) increased. Even though these extracts exhibited their effects positively on the various biochemical parameters, the degummed seed oil extract’s effects were to some extent more pronounced compared to the crude seed oil extract’s effects.


Keyword: Citrullus lonatus, L- ascorbic acid, carbon tetrachloride, haematological indices, crude and degummed seed oil extracts


 Received: July 30, 2020  Accepted: August 15, 2020  Published: August 31, 2020

Bello And Tijwun


Copyright © 2020 Direct Research Journal of Health and Pharmacology