Article Number: DRJHP30673164

DOI: https://doi.org/10.26765/DRJHP30673164

ISSN: 2449-0814

Vol.9, Pp. 20-27, 2021

Copyright © 2021

Author(s) retain the copyright of this article


Original Research Article

In vivo assessment of anti-plasmodial properties of the methanolic extracts of Morinda lucida, on albino mice experimentally infected with Plasmodium berghei berghei

LeBari Barine Gboeloh*

Roseline Ogechi Ofuru

Kingsley Elele


Abstract

The effects of the methanolic extracts of the leaves, stem and roots of Morinda lucida at various concentration on albino mice experimentally infected with Plasmodium berghei berghei was investigated. Forty-eight albino mice used were divided into twelve groups of four mice each. The groups were designated L (Lo, L1, L2), S (S1, S2, S3), R (R1, R2, R3) X, Y, and Z.  All animals in groups L, S, and R were infected with Plasmodium berghei, while Groups X, Y, and Z served as the control. Group X was fed with food and water only, Y was infected but not treated while group Z was infected and treated with chloroquine.  Six days after inoculation of mice with the parasite, groups Lo, L1, L2, S1, S2, S3 and R1, R2, R3 were treated orally with 100,300 and 500mg/kg body weight of the methanolic extract of the plant parts respectively for five days. Group Z was treated with 5mg/kg body weight of chloroquine. The mice parasitized with P. berghei berghei were euthanized after six days of having shown clinical signs of malaria. The phytochemical analysis of the plant showed the presence of alkaloid (stem: 2.88±0.35, root: 4.07±0.06, leaf: 8.51±0.22), Tannin (Stem: 2.48±0.35, root: 2.48±0.35, leaf: 1.94±0.21), Phenol (stem: 0.88±0.10, root: 0.79±0.05, leaf: 0.61±0.03), Flavoid (stem: 6.94±0.77, root: 2.68±0.23, leaf: 3.25±0.29) and saponin (stem: 26.81±0.40, root: 20.27±0.89,  8.43±0.54). The Plasmodium suppressive test indicated that all concentrations (100, 300, and 500mg/kg body weight) of the methanolic extracts of the plant parts had a significant (P<0.05) suppressive effect on the parasite. At the highest dose of 500mg/kg body weight, inhibition rates of 92.75±0.09, 90.75±0.21, and 94.38±1.09 were recorded for the roots, stems, and leaves respectively. This was also significant when compared with the 99.8±1.00 inhibition rate exhibited by the standard drug (Group Z).  The results of the effect of the plant extracts on some hematological indices showed a mean value dose-dependent increase in the levels of PCV, Hb, NEU, and LYMP. The plant parts of M. lucida possess antiplasmodial effects against Plasmodium berghei berghei as shown by the percentage parasite clearance. The plant also posses hematopoietic, hepatoprotective, and renalgesic effects.


Keywords:  Morinda lucida, methanolic extracts, antiplasmodial effect, Plasmodium berghei berghei, haematological indices


 Received: March 16, 2021  Accepted: April 18, 2021  Published: April 26, 2021

Gboeloh Et Al


Copyright © 2021 Direct Research Journal of Health and Pharmacology