Article Number: DRJHP098321785


ISSN: 2449-0814

Vol.9, pp. 1-9, 2021

Copyright © 2021

Author(s) retain the copyright of this article

Original Research Article

Orogastric sub-acute codeine administration induced hepatotoxicity in adult albino wistar rats

Ayuba, Y.*

Ibrahim, I.

Bala, G. M.


This study was on orogastric sub-acute codeine administration induced hepatotoxicity in adult albino Wistar rats. The objectives of the study were; to determine the effects of codeine on liver function in adult albino Wistar rats and to investigate the effects of codeine on the histology of the liver in adult albino Wistar rats at the light microscopic level. The animals were divided into 4 groups of 5 rats each, Groups A, B, C, and D. Group A was the control group and given normal feed and water ad libitum for 28 days. Group B rats were the low dose group and were administered codeine 30mg/kg by orogastric gavage daily for 28 days in addition to normal feed and water ad libitum. Group C rats were the medium-dose group and were administered 60mg/kg codeine. Group D rats were the high dose group and were administered 90mg/kg codeine for 28 days. Ethical clearance was obtained from the animal ethical committee, Bayero University Kano for the study. Data obtained from this study were calculated for one-way analysis of variance (ANOVA), followed by student t-test. Results were expressed as mean ± standard error of the mean. Computer statistical software package SPSS version 20 was used for data analysis. All statement of differences was based on significance at p<0.05. Twenty male and female albino rats weighing between 150-200g were used for the experiment. Results from this study showed that alanine aminotransferase (ALT) and aspartate aminotransferase (AST) slightly increased but this increase was not statistically significant while the increase observed in alkaline phosphatase (ALP) was statistically significant at p<0.05. The histopathology of the liver showed normal hepatocytes radiating from the central vein and clear sinusoids in group A the control. The micrograph of the treated groups (B, C, and D) showed focal pale staining, hepatic necrosis, and inflammatory cells infiltration, focal aggregation of mononuclear cell infiltration, multi-focal fatty tissue, binuclear cell infiltration, mild vacuolation, focal enlarged and congested blood channel and presence of giant cells. This study concludes that codeine causes a significant increase in ALP activity and hepatotoxicity in albino Wistar rats.

Keywords: Albino rats, codeine, hepatotoxicity, orogastric,sub-acute

 Received: January 6, 2021  Accepted: February 8, 2021  Published: February 28, 2021

Ayuba Et Al

Copyright © 2021 Direct Research Journal of Health and Pharmacology