Abstract

The study was undertaken to investigate the effect of pH and bacterial count using on biogas generation using Cattle rumen content at different retention time. The bacterial associated with the production as well as the pH of the slurry before and after the biogas production was determined. From the result, bacteria colony count range from (2.08×10⁷cfu/ml to 4.2×10⁷cfu/ml). Isolate identified from biogas generation of spent slurry are Yersinia entrocolitica bacillus lintus, Bacillus lichinoformis, Bacillus firmus,Escherichia coli, Pseudomonas aeruginosa, Starphylococcus carpitis, Bacillus alvei, Bacillus pathenticus, Salmonella sp, Bacillus sp and Proteus vulgarius the highest volume of biogas generation increase with time up to a maximum of (2240 cm³) at the end of second week at rate of (93.3) per hour, while the least volume (620 cm³) was obtained in week one. The optimum pHs of the digester before and after biogas generation were 6.3 and 7.2 respectively. The most frequently encounter bacteria isolated from the spent slurry is Bacillus sp. which account for (49.8%). The study entails that bacterial isolated from spent slurry are essential for biogas generation. Government should train expert in the biogas generation and developed researcher on biogas using cattle rumen at different retention time.