Original Research Article
Phytochemical Screening, Chromatographic Evaluation and Antibacterial Activity of the Leaf Extracts of Lawsonia Inermis L
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Article Number: DRJBB432794316
DOI: https://doi.org/10.5281/zenodo.3246786
ISSN: 2734-2158
Vol.5 (2), pp. 9-16, June 2019
Copyright © 2019
Author(s) retain the copyright of this article
Abstract
The crude methanolic extract of Lawsonia inermis leaves was subjected to chromatographic separation by column and thin layer chromatography techniques. Hexane, ethyl acetate, and methanol were used as solvent system. The eluted fractions of the preliminary column chromatography gave eleven (11) fractions. All the fractions had distinct colours ranging from green to reddish brown. The eluted fractions were spotted on TLC and six brands were yielded (LT-1, LT-2, LT-3, LT-4, LT-5, LT-6). The eluted fractions were screened for antibacterial activity. The eluted fractions, LT-2 – LT-6 exhibited some degree of antibacterial activity. Furthermore, the eluted compound fractions were assayed for minimum inhibitory concentration (MIC) using the 96-well microdilution technique. Phytochemical screening of the extract revealed the presence of alkaloids, flavonoids, saponins, phenols, tannins, steroids and anthraquinone.
Keywords: Column chromatography, Lawsonia inermis, minimum inhibitory concentration, phytochemical screening, thin-layer chromatography.Received: April 18, 2019 Accepted: June 9, 2019 Published: June 20, 2019