Key words: Antibacterial activity, bacteria, photochemical, salmonella typhimurium, cassia occidentalis"> Antibacterial activity of the aqueous leaf extract of cassia occidentalis (sanga-sanga) on salmonella typhimurium – Direct Research Journal of Biology and Biotechnology
Antibacterial activity of the aqueous leaf extract of cassia occidentalis (sanga-sanga) on salmonella typhimurium Antibacterial activity of the aqueous leaf extract of cassia occidentalis (sanga-sanga) on salmonella typhimurium – Direct Research Journal of Biology and Biotechnology
Original Research Article

Antibacterial activity of the aqueous leaf extract of cassia occidentalis (sanga-sanga) on salmonella typhimurium

1Usman, A. A.

*2Bello, A.

4Salihu, A. A.

3Ezeamagu, C. E.

5Odeh, A. P.

6Zainab, A. B.

Article Number: DRJA10147593
DOI: https://doi.org/10.26765/DRJBB.2017.7593
ISSN: 2734-2158

Vol. 3 (5), pp. 74-80, December 2017

Copyright © 2017
Author(s) retain the copyright of this article


Abstract

The Cassia occidentalis leaves was obtained from Usmanu Danfodiyo University Sokoto, Fish farm and it was authenticated at Usmanu Danfodiyo University herbarium after it was taken to Microbiology Laboratory Usmanu Danfodiyo University, Sokoto for aqueous extraction Fifty grams of grounded leaves was dissolved in 500 ml of distilled water and the extract was evaporated in hot air oven at 40°C for 9 days. The concentrations were tested against S. typhi that was obtained from specialist Hospital Sokoto (SHS) after the test isolate was tested for viability and confirmed at the Microbiology Laboratory Usmanu Danfodiyo University, Sokoto. The leaf extract was tested at different concentrations of 20 mg/ml, 40 mg/ml, 60 mg/ml, 80 mg/ml and 100 mg/ml. But there was no activity recorded at 20 mg/ml. And the minimum inhibitory concentration (MIC) was recorded at 30 mg/ml and minimum bactericidal concentration (MBC) was recorded at 60 mg/ml. The phytochemical screening reveals the presence of Flavonoid (++), Tannin (+), Saponin (+++), Glycoside (+), Cardiac glycoside (+), Steroid (+), Saponin glycoside (+), Anthraquinones (+), and Volatile oil (+). The presence of these photochemical may be attributed to the antibacterial activity of the extracts.

Key words: Antibacterial activity, bacteria, photochemical, salmonella typhimurium, cassia occidentalis
 Received: November 20, 2017  Accepted: December 8, 2017  Published: December 12, 2017



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