Article Number: DRJBB432794316


ISSN: 2734-2158

Vol.5 (2), pp. 9-16, June 2019

Copyright © 2019
Author(s) retain the copyright of this article

Original Research Article

Phytochemical Screening, Chromatographic Evaluation and Antibacterial Activity of the Leaf Extracts of Lawsonia Inermis L

*Onuh, O. A.

Machunga-Mambula, S. S.

Akin-Osanaiye, B. C.


The crude methanolic extract of Lawsonia inermis leaves was subjected to chromatographic separation by column and thin layer chromatography techniques. Hexane, ethyl acetate, and methanol were used as solvent system. The eluted fractions of the preliminary column chromatography gave eleven (11) fractions. All the fractions had distinct colours ranging from green to reddish brown. The eluted fractions were spotted on TLC and six brands were yielded (LT-1, LT-2, LT-3, LT-4, LT-5, LT-6). The eluted fractions were screened for antibacterial activity. The eluted fractions, LT-2 – LT-6 exhibited some degree of antibacterial activity. Furthermore, the eluted compound fractions were assayed for minimum inhibitory concentration (MIC) using the 96-well microdilution technique. Phytochemical screening of the extract revealed the presence of alkaloids, flavonoids, saponins, phenols, tannins, steroids and anthraquinone.

Keywords: Column chromatography, Lawsonia inermis, minimum inhibitory concentration, phytochemical screening, thin-layer chromatography.

 Received: April 18, 2019  Accepted: June 9, 2019  Published: June 20, 2019

Onuh Et Al

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