Article Number: DRJBB214335658

DOI: https://doi.org/10.5281/zenodo.3246793

ISSN: 4372-2608

Vol.5(2), pp.17-23, June 2019

Copyright © 2019
Author(s) retain the copyright of this article


Original Research Article

Phytochemical Screening, Chromatographic Evaluation and Antibacterial Activity of the Leaf Extracts of Mitragyna Inermis (Willd)

*Onuh, O. A., 

Machunga-Mambula, S. S.

Akin-Osanaiye, B. C.


Abstract

Mitragyna inermis is a plant used in traditional medicine in Nigeria against bacterial infections, infectious diseases to mention a few. Dried crude methanolic extract of M. inermis leaf was fractionated successively using hexane, ethyl acetate and methanol as solvent systems. The column fractionation yielded eleven fractions (MC1 – MC11) while the thin-layer chromatography yielded six visible bands (MT-1 – MT-6). Antimicrobial activity of the fractions showed that the plant had some inhibitory effect against the test organism. The photochemistry of the eluted fractions showed varied contents of alkaloids, tannins, saponins, flavonoids, alkaloids and glycosides.


Keywords: Chromatography, fractionation, Mitragyna inermis


 Received: April 18, 2019  Accepted: June 9, 2019  Published: June 20, 2019

Onuh Et Al 1


Copyright © 2019 Direct Research Journal of Biology and Biotechnology